Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways.Objective To study the effect and mechanism of gene PBP2 and gene PBP3 in drug resistance of Klebsiella pneumoniae(KP) to different antibacterial agents. Proteomics SequenceĬomprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Interaction between FtsW and penicillin-binding protein 3 (PBP3) directs PBP3 to mid-cell, controls cell septation and mediates the formation of a trimeric complex involving FtsZ, FtsW and PBP3 in mycobacteria. Structure of Mycobacterium tuberculosis FtsZ reveals unexpected, G protein-like conformational switches. Homolog Mutant FunctionĬomprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. MutantĬonditional expression of Mycobacterium smegmatis ftsZ, an essential cell division gene. Genes required for mycobacterial growth defined by high density mutagenesis. Physiological consequences associated with overproduction of Mycobacterium tuberculosis FtsZ in mycobacterial hosts. Regulation of hmp gene transcription in Mycobacterium tuberculosis: effects of oxygen limitation and nitrosative and oxidative stress. Check for mutants available at TARGET website Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. MRNA identified by Northern blotting analysis maximal level of transcription at log-phase growth (see Hu et al., 1999).Įssential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. Translational start site supported by proteomics data (See Kelkar et al., 2011).
tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the membrane fraction of M. FtsW|Rv2154c interacts with PbpB|Rv2163c and FtsZ|RvRv2150c (See Datta et al., 2006). FASTA best: FTSZ_STRCO P45500 cell division protein FtsZ (399 aa) opt: 1674, E(): 0 (77.3% identity in 339 aa overlap). Contains FtsZ protein signature 2 (PS01135). FtsZ, cell division protein (see Dziadek et al., 2002). It is thought that the intracellular concentration of FTSZ protein is critical for productive septum formation in mycobacteria.
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